Fig. 3

Restoration of PRELP gene expression by HDAC inhibitors in bladder cancer cells. RT-qPCR analyses of PRELP (gray bars) gene expression in RT4 (A) and J82 cells (B). The cells were treated with either DMSO or increasing concentration of BIX01294 (0, 0.625, 1.25, 2.5, 5, and 10 μM), UNC0638 (0, 0.625, 1.25, 2.5, 5, and 10 μM), EPZ011989 (0, 0.625, 1.25, 2.5, 5, and 10 μM), UNC1999 (0, 0.625, 1.25, 2.5, 5, and 10 μM), 5-azacytidine (0, 0.625, 1.25, 2.5, 5, and 10 μM), TSA (0, 0.25, 0.5, 1.0, and 2 μM), and SAHA (0, 0.3125, 0.625, 1.25, 2.5, and 5 μM) for 72 h. Data are presented as the mean ± standard deviation (SD) of three technical replicates. The x-axis shows the fold increase relative to DMSO-treated control. The y-axis shows the name and concentration of each inhibitor. The red line indicates a fold increase of 1