Fig. 4

PCDHB15 overexpression in WM266-4 cells impairs 3D cell invasion. The invasion ability of WM266-4 cells (WT), control cells (mock) and PCDHB15-overexpressing cells (clones 8, 12 and 13) was measured using a 3D invasion assay in collagen matrix. Images are representative of at least 6 spheroids per condition before (A) and after (B) 24 h invasion. The initial sizes of each spheroid (C) and their invasion index at 24 h (D) are reported as histograms. Means and SEM were calculated from 6 spheroids measured in three independent experiments. Jarque–Bera’s test to analyze normality, Fisher’s test to analyze variances and Student t test were performed; p value < 0.05, **: p value < 0.01. ***: p value < 0.001