Fig. 3
From: Epigenetically regulated PCDHB15 impairs aggressiveness of metastatic melanoma cells
3D aggregation of WM266-4 cells is impaired by PCDHB15 overexpression. The formation of aggregates of WM266-4 cells (WT), control cells (mock) and PCDHB15-overexpressing cells (clones 8, 12 and 13) was monitored by bright-field time-lapse video microscopy. A The images show representative aggregates at 0, 5, 15 and 20 h after the experiment onset. Pink lines delineate the maximal aggregate areas. Green lines delineate empty areas that are subtracted in the total area calculation. B The normalized area of the aggregates is reported at each time. The reported values are the mean of at least 6 individual aggregates analyzed in three independent experiments