Fig. 3

Annotation of differentially methylated CpG loci in T-LGLL with chromatin states of CD8⁺ memory cells. A Differentially hyper- or hypomethylated CpG loci between T-LGLL and normal T-cell subsets were annotated by the chromatin segmentation data of CD8⁺ memory cells from the BLUEPRINT project (chromatin segmentations E1–E11 are depicted in different colors in %, color code explained in Additional file 1: Table S17). The first “background” bar represents the loci of the array. From left to right the following CpG subsets are shown: (1) All 450 k CpGs, annotated to chromatin states (2) Significant hypermethylated CpGs in T-LGLL samples vs non-neoplastic T cells, (3) significant. hypomethylated CpGs in T-LGLL samples vs non-neoplastic T cells, (4) significant hypermethylated CpGs located in promoters or enhancers (as defined by the Illumina 450 k annotation, see Additional file 11) in T-LGLL samples vs non-neoplastic T cells, (5) significant hypomethylated CpGs located in promoters or enhancers (as defined by the Illumina 450 k annotation, see methods) in T-LGLL samples vs non-neoplastic T cells. B Chromatin state enrichment of CD8-positive memory genome regions containing significantly differentially methylated CpG loci (hyper and hypomethylated, bar 2 and 3, Fig. 3A, respectively) compared to 450 k background CpG loci (bar 1, Fig. 3A). (*) Significant enrichments (hypergeometric test, p value < 0.01). Chromatin segmentation E1–E11 is described in Additional file 1: Table S17