Table 3 Effects of ovarian tissue cryopreservation on imprinted genes
Reference | Species | Materials | Technology of assessment | Targeted imprinted genes | Conclusions |
|---|---|---|---|---|---|
Sauvat et al. [119] | mouse | ovarian tissue | Southern blotting | H19 and Kcnq1ot1 | No significant differences |
He et al. [120] | mouse | ovarian tissue | Western blot and q-PCR | Grb10 | Grb10 was significantly increased after vitrification |
Wang et al. [121] | mouse | ovarian tissue | Bisulfite sequencing PCR | Snrpn | Freezing of ovarian tissue did not affect the methylation status of Snrpn-DMR of imprinted genes in GV oocytes |
Trapphoff et al. [122] | mouse | pre-antral follicles | pyrosequencing | H19, Igf2 and Snrpn | The methylation of DMR of imprinted gene H19 and Igf2r in mature oocytes was not affected, and Snrpn changed slightly |