Fig. 1

Overview of the study and selection of WID™-qCIN MethyLight reactions. A Overview of the study setup. B Example plots of CpG methylation beta values in cervical samples of controls and cervical intraepithelial neoplasia grade 3+ (CIN3+) cases versus immune cell proportion (ic) and C mean methylation values of CpGs in close genomic proximity (within 500 base pairs) to the index CpG, to identify differentially methylated regions. D AUC of individual MethyLight reactions for discrimination of (current or future) CIN3 + cases from controls in the LBC-CIN Discovery set. Three reactions were selected for the WID™-qCIN test using a minimal conditional mutual maximisation filter for feature selection (maximum information with the outcome, minimal redundancy with each other). CIN cervical intraepithelial neoplasia, LBC liquid-based cytology