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Fig. 4 | Clinical Epigenetics

Fig. 4

From: PDCD5 inhibits progression of renal cell carcinoma by promoting T cell immunity: with the involvement of the HDAC3/microRNA-195-5p/SGK1

Fig. 4

RCC proliferation is curbed, and T cell activation is facilitated by miR-195-5p through targeted inhibition of SGK1. A The intersection Venn diagram of downstream target genes of miR-195-5p predicted by miRDB, StarBase, TargetScan, and mirDIP. B The intersection Venn diagram of upregulated differentially expressed genes from GSE100666 dataset and candidate target genes. C The expression box diagram of SGK1 in RCC cancer samples from the GSE100666 dataset. D The expression of SGK1 in RCC and adjacent non-cancerous tissues determined by Western blot analysis (n = 58). *p < 0.05 versus the adjacent non-cancerous tissues. E The expression of SGK1 in the RCC cell line (A498) and normal renal cell line (HK-2) normalized to GAPDH determined by Western blot analysis. *p < 0.05 versus the HK-2 cell line. F Targeting relationship between miR-195-5p and SGK1 determined with TargetScan. G Relative luciferase activity measured by the dual-luciferase reporter gene assay. *p < 0.05 versus the NC mimic group. H miR-195-5p expression in A498 cells determined by RT-qPCR. *p < 0.05 between the two groups. I mRNA expression of SGK1 in A498 cells after transfection determined by RT-qPCR. *p < 0.05 between the two groups. J and K The protein expression of SGK1 normalized to GAPDH determined by Western blot analysis following different treatments. *p < 0.05 between the two groups. L A498 cell proliferation detected by EdU assay. *p < 0.05 between the two groups. M IFN-γ+ T cell proportion and CD3+ T cell proliferation detected by flow cytometry. *p < 0.05 between the two groups. N The expression of IFN-γ released from CD3+ T cells determined by ELISA. *p < 0.05 between the two groups. The results were measurement data and expressed as mean ± standard deviation. The results were collected from at least 3 independent cell experiments. Data between RCC and adjacent non-cancerous tissues were analyzed by paired t test, and data comparison between other the two groups was analyzed by unpaired t test

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