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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Regional gain and global loss of 5-hydroxymethylcytosine coexist in genitourinary cancers and regulate different oncogenic pathways

Fig. 5

Quantification of the stemness-associated 5hmC gain with a malignant signature. A Venn diagram showing 30 genes obtaining increased 5hmC and expression in prostate tumors and 3D-cultured prostate tumor cells. Seven genes are highlighted for being PFS-related in the TCGA PRAD cohort. B Pearson’s correlation analysis showing the malignant signature is negatively associated with the hypo-hydroxymethylation signature and vice versa. C Kaplan–Meier plot of the relationship between patient PFS and the malignant signature score within the TCGA PRAD cohort. Patients were stratified by the score median. P values were produced using log rank test. D Violin plots showing estimated malignant signature scores of patients stratified by the Gleason score. E UMAP projections of 23,674 prostate cancer cells, color-coded by subtypes. F UMAP visualization of the Scissor+ cells which are predicted to possess a higher malignant signature. G Bar plot depicting the fraction of Scissor+ cells in the 3 subtypes of prostate tumor cells. H Violin plots showing differences in the malignant signature (left) and the adult stem cell signature (right) between the Scissor+ and Scissor− populations. I. GSEA displaying enriched gene sets in Scissor+ cells. In (D) and (H) P values were produced with t test. In (B), (C), (E) and (F), error bars represent mean ± standard deviation. P values were produced with Mann–Whitney U test.

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