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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Regional gain and global loss of 5-hydroxymethylcytosine coexist in genitourinary cancers and regulate different oncogenic pathways

Fig. 1

Genome-wide 5hmC profiling of the genitourinary system in heath and cancer. A Schematic diagram showing the genome-wide 5hmC sequencing of healthy and cancerous genitourinary tissues. B Meta-gene plot of 5hmC profiles in genitourinary tissues. The color range indicates log2 (TPM + 1) values. TSS, transcription start site. TES, transcription end site. C Dot blot showing distinct 5hmC levels in normal genitourinary tissues including the kidney, the prostate and the urothelium (top). Methylene blue (MB) staining was used as a DNA loading control (bottom). D Bar plot displaying numbers of total 5hmC peaks identified in normal genitourinary tissues and genitourinary tumors. E Venn diagrams showing overlapping normal-specific peaks in genitourinary tissues (left) and the overlap of tumor-specific 5hmC peaks (right). The intersections are peaks common to healthy genitourinary tissues (left) and genitourinary tumors (right). F Gene Ontology (GO) analysis of the common 5hmC peaks. G Genome browser tracks depicting the 5hmC level of WNT7B in healthy and cancerous genitourinary tissues including the kidney, the prostate and the urothelium. H Bar plot showing the 5hmC level of WNT7B measured by hMeDIP-qPCR in matched normal and tumor samples of the kidney, the prostate and the urothelium. Primers were designed at the positions indicated by red boxes in (G). Error bars represent mean ± standard deviation. P values were produced with t test

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