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Fig. 3 | Clinical Epigenetics

Fig. 3

From: The absence of AhR in CD4+ T cells in patients with acute graft-versus-host disease may be related to insufficient CTCF expression

Fig. 3

Insufficient CTCF decreases AhR in aGVHD CD4+ T cells. A Schematic diagram of CTCF binding sites in the AhR promoter region. B ChIP-PCR showed that CTCF binds in the AhR promoter region (− 204 bp to + 611 bp). C ChIP-qPCR analysis of CTCF enrichment in the AhR promoter in chromatin fractions extracted from CD4+ T cells from patients with aGVHD (n = 15) and the non-aGVHD group (n = 15). Data are relative to input DNA prepared from untreated chromatin. D Correlation between CTCF enrichment and AhR mRNA levels in aGVHD CD4+ T cells (n = 15). E–G CTCF and AhR expression levels in aGVHD CD4+ T cells after CTCF expression (n = 3). E Relative mRNA levels. F Representative Western blot results. G Quantitative analysis of band intensities. Data represent the means of three independent experiments. H–J CTCF and AhR expression levels in normal CD4+ T cells after CTCF silencing (n = 3). H Relative mRNA levels. I Representative Western blot results. J Quantitative analysis of band intensities. Data represent the means of three independent experiments (*P < 0.05, **P < 0.01)

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