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Table 3 PCR program used for RT-qPCR

From: Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line

95 °C

03:00 min

 

95 °C

00:10 min

40 x

60 °C

00:20 min

72 °C

00:30 min

Melt

00:15 min