Skip to main content
Fig. 3 | Clinical Epigenetics

Fig. 3

From: Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line

Fig. 3

Monitoring of cell death event of MOLM-13 after treatment with AzaC or AzadC. a Flow cytometric analysis of cell death after 72 h treatment with either AzaC or AzadC in different concentrations. Cells that were Annexin V low and Sytox™ low cells were considered viable, cells only high in Sytox as necrotic, cells only high in Annexin V as early apoptotic and cells high in both as late apoptotic. b Quantification of three independent flow cytometric cell death analyses performed as depicted in (a) was applied, combining all Annexin V high cells as total apoptotic. c Differential analysis of early and late apoptotic events of b. d Immunoblot analysis of BCL-2 protein levels upon AzaC and AzadC treatment (2.5 µM each for 72 h) as compared to untreated control. Histone H3 served as a loading control. b, c Each dot represents one independent experiment. Bars show mean, and error bars represent standard deviation. Ordinary one-way ANOVA combined with Dunnett’s multiple comparisons test was performed within each observed group (viable and apoptotic in b, early and late apoptotic in c). All p values were adjusted for multiple comparisons testing. ns padj ≥ 0.05, *padj < 0.05, **padj < 0.01, ***padj < 0.001, ****padj < 0.0001. b Details about the analysis, including exact p values, are given in Additional file 13: Table S12. c: Details about the analysis, including exact p values, are given in Additional file 14: Table S13

Back to article page