Fig. 7
CRNDE enhances sorafenib resistance through upregulation of EGFR with p300 in HCC. A Huh7 cells from pcDNA3-control and CRNDE overexpression groups were incubated with or without sorafenib and assessed via the MTT assay. B Nude mice were subcutaneously injected with pcDNA3-control or CRNDE overexpression Huh7 cells, followed by DMSO (vehicle control), C646 or sorafenib treatment, and tumor growth monitored. Tumor sizes (cm) are indicated at the bottom. C Chart showing tumor sizes and weights from B. D, E Survival rates of nude mice from B. Kaplan–Meier plots using the standard tumor size (200 mm3) as a criterion for euthanasia from pcDNA3-control and CRNDE overexpression groups. F, G qRT-PCR and western blot analyses of EGFR mRNA and protein expression in tumors of mice. H Working model of the function of the CRNDE/p300/H3K9Ac/YY1 complex in regulation of EGFR expression. Statistical significance (p value) was calculated with the two-tailed Student's t-test for a single comparison between two groups. Data are presented as mean ± SD (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)