Fig. 6
CRNDE promotes CDX tumor growth in mice via p300/YY1/H3K9Ac regulation of EGFR. A, B Huh7 and J7 cells were transfected with pcDNA3-control or pcDNA3-CRNDE plasmids with shRNA-silenced p300. Cell numbers were assessed with the proliferation assay. C, D Huh7 and J7 cells from pcDNA3-control and CRNDE overexpression groups were incubated with or without C646 and evaluated via the MTT assay. E Nude mice were subcutaneously injected with pcDNA3-control or CRNDE overexpressing J7 cells followed by vehicle control (DMSO) or C646 treatment and tumor growth monitored. Tumor sizes (cm) are indicated at the bottom. F, G Chart showing tumor sizes and weights from E. H Kinetic changes in tumor sizes of CDX following C646 treatment. I Survival rates of nude mice from E. Kaplan–Meier plots using standard tumor sizes (150 mm3) as a criterion for euthanasia. J qRT-PCR analysis of EGFR mRNA in tumors of mice. K Determination of EGFR protein expression in mouse tumors via immunohistochemistry. Positive staining (brown) in tumor tissues. Size reference (10 μm) is indicated. Statistical significance (p value) was calculated with the two-tailed Student's t-test for a single comparison between two groups. Data are presented as mean ± SD (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)