Fig. 1
From: Genomic targets and selective inhibition of DNA methyltransferase isoforms
Construction of DNMT overexpressing cell lines. A Diagrammatic representation of isoforms from the DNMT3A, DNMT3B, DNMTΔ3B and DNMT3L subfamilies. Exons are indicated, with missing or excluded exons in grey. Also indicated are the proline-tryptophan-tryptophan-proline (PWWP) domain (blue), cysteine-rich PHD zinc finger domain (green), and C-terminal catalytic domain (red). DNMT1 is not illustrated due to its highly divergent N-terminal region. B Bright field and fluorescence microscope images of HEK293T cells transduced with the DNMT3L construct, expressing GFP. C Selection of GFP-positive (pink) transduced cells by FACS. D Morphology of single-cell colonies grown in cells transduced to overexpress DNMTΔ3B4. E Exogenous expression of DNMT3B1-5 in transduced cells, relative to PPIA and GAPDH. Data are expressed as means ± SD