Table 2 Different biological nanopores characteristics. Adapted from [142]
From: Epigenetic tumor heterogeneity in the era of single-cell profiling with nanopore sequencing
Biological nanopore | Diameter (nm) | Analyte | Comment |
|---|---|---|---|
α-HL | 1.4 | RNA, ssDNA, aa, polymers, peptides, proteins | Large-scale application due to its reproducible structure and easy manipulation by site-directed mutagenesis |
MspA | 1.2 | ssDNA, dsDNA | Suitable geometry for nanopore DNA sequencing |
Phi 29 | 3.6 | ssDNA, dsDNA | Allowing for the detection of large analysts and offering more space for further modifications |
ClyA | 3.3 | ssDNA, proteins | Suitable for the accommodation of small to medium-sized proteins within the nanopore lumen |
FhuA | 2.4 | Enzymes, protein–DNA interaction | Examining the proteolytic activity of an enzyme at pH 3.9 and determining the kinetics of protein–DNA–aptamer interactions |
Aerolysin | 1–1.7 | Peptides, proteins | Sensing the α-helix peptides and unfolded proteins |
SP1 | 3 | ssDNA | Analyzing of ssDNA |