Table 1 Comparison of different NGS technologies. Adapted from [36]
From: Epigenetic tumor heterogeneity in the era of single-cell profiling with nanopore sequencing
| Â | Roche 454 | Illumina/Solexa genome analyzer | PacBio SMRT | MinION |
|---|---|---|---|---|
Company | Roche | Illumina | Pacific Biosciences | Oxford Nanopore Technologies (ONT) |
Release year | 2005 | 2006/7 | 2010 | 2014 |
Method | Sequencing by synthesis | Sequencing by synthesis | Sequencing by synthesis | Direct sequencing |
Price | $13,700 | Expensive—$ 49,000 to over a million | $695 | $1000 with 500 disposable flow cells |
Average reads | 100–150 bp | 75 bp highly accurate | 10-25 Kb | The longest reads over 100 Kb |