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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Epigenetic tumor heterogeneity in the era of single-cell profiling with nanopore sequencing

Fig. 2

Principle of single-molecule real-time sequencing. A A single molecule of DNA template-bound Phi 29 is immobilized at the bottom of the zero-mode waveguide nanophotonic structure, which is illuminated by laser light. B Diagrammatic order of the phospho-linked dNTP association cycle. (1) Phospho-linked nucleotide forms a binding with a template in the polymerase active site. (2) Advancing fluorescence output on the analogous color channel. (3) Phosphodiester linkage formation releases the dye binder phosphate product followed by the ending of zero-mode waveguide nanophotonic fluorescence pulse. (4) Translocation of polymerase enzyme to the next nucleotide of the template strand. (5) Binding of the next cognate nucleotide on the active site of polymerase to continue the cycle [57, 58]. Image reprinted from [57] with permission of the publisher (Order license ID: 1164215-1, 25 Nov 2021)

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