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Fig. 3 | Clinical Epigenetics

Fig. 3

From: JIB-04, a histone demethylase Jumonji C domain inhibitor, regulates phenotypic switching of vascular smooth muscle cells

Fig. 3

JIB-04 regulated G2/M phase gene expression and induced cell cycle arrest. A-B. Flow cytometry analysis showed the ratio of HASMCs in different cell cycle phases after treatment with DMSO or JIB-04 (0.5 μM) for 48 h (n = 3 per group). C. Quantification of the mRNA levels of CCNB1, CCNB2, CDK1, CCNA1, CDC25C, PLK1, and WEE1 in HASMCs after JIB-04 treatment (n = 4 per group). D-E. Protein levels of phosphorylated CDC2 (p-CDC2), phosphorylated RB (p-RB), phosphorylated CHK1 (p-CHK1), and phosphorylated CHK2 (p-CHK2) in HASMCs after JIB-04 treatment (n = 4 per group). β-Actin served as a loading control. *P < 0.05, **P < 0.01, N.S. no significant

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