Fig. 4

Catalytic P300/CBP inhibition stymies IRF1-dependent transactivation of IFNγ-induced genes. A Column-normalized heatmap of gene expression by RNA-seq for IFNγ stimulated genes in the presence of IFNγ ± A-241. B AT3 cells stimulated with IFNγ ± A-241 for 3 h prior to immunoblot for Tubulin, STAT1, or phosphor-STAT1 (Y701). C Acetylation of H3K27 by ChIP-seq at IFNγ-induced loci following IFNγ ± A-241. D IGV screenshot of Trim21, Igtp, and Cd274 loci showing H3K27ac ChIP-Rx, ATAC-seq, and IRF1 ChIP-seq in the presence of IFNγ ± A-241. E Binding of IRF1 by ChIP-seq to IFNγ-induced loci following IFNγ ± A-241. F Chromatin accessibility by ATAC-seq at IFNγ-induced loci following IFNγ ± A-241. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, Mann–Whitney U test