Fig. 5

Validation of specific methylation-based markers in in-house study. A Validation of the methylation markers using target bisulfite sequencing array in validation cohort 3 and cfDNA pilot cohort. Numbers represent average methylation levels of CpG sites in multiple specimens. B and C Validation of the methylation markers using Droplet Digital PCR in cfDNA validation cohort. **P < 0.05. D ROC curves for tissue-specific methylation markers in the cfDNA validation cohort