Maternal–fetal stress and DNA methylation signatures in neonatal saliva: an epigenome-wide association study

Clinical Epigenetics

Table 2 CpG sites associated with stress measures in DNA methylation analysis

Stress measure	Probe	Coef^d	P.Value^e	FDR^f	chr^g	Illumina gene annotation	Genes within 50 kb of associated CpG
PSS^a	cg17478679	−0.2	3.59E-07	0.25	chr17	KPNB1	CRHR1
	cg22124215	−0.1	1.07E-06	0.25	Chr2	MARCH4	DIRC3
	cg06195987	0.52	1.96E-06	0.25	Chr7	NA	LMTK2
	cg15426815	0.31	2.24E-06	0.25	Chr12	MIR200C	C1S
PDQ^b	cg06542869	0.02	4.62E-08	0.03	chr11	YAP1	YAP1
	cg22861369	0.02	2.30E-07	0.08	chr5	PDLIM4	SLC22A4
	cg01629131	0.03	3.31E-07	0.08	chr20	NA	RP11, RP1
	cg03105159	0.01	7.18E-07	0.11	chr2	ALKAL2	ALKAL2, FAM105B
Cortisol	cg11409463	0.003	2.87E-09	0.002	chr5	NA	SCAMP1
	cg20905655	0.004	1.16E-07	0.03	chr19	NA	SSBP4;
	cg25252839	0.002	1.24E-07	0.03	chr1	TOMM20	SNORA14B
	cg05306225	−0.002	2.08E-07	0.04	chr8	CSMD1	CSMD1
FSI^c	cg13547817	−0.39	8.85E-08	0.07	chr9	ERP44	ERP44; INVS
	cg07642729	−0.35	2.81E-06	0.49	chr8	ASB15	-
	cg24795351	−0.34	3.79E-06	0.49	chr8	PREX2	PREX2
	cg16692227	−0.21	3.80E-06	0.49	chr14	SAMD12	SAMD12

The table shows top four CpGs from the EWAS that are associated with the respective stress measures. Marked in bold are significant
^aCohen Perceived Stress Scale
^bPrenatal Distress Questionnaire
^cFetal Stress Index
^dRegression coefficients from the statistical model
^eSignificance from the statistical model
^fFalse discovery rate
^gChromosome
NA: Not available

ISSN: 1868-7083