Fig. 5
From: Targeted demethylation of the SLC5A7 promotor inhibits colorectal cancer progression
Targeted demethylation of SLC5A7 influence of p53 and its downstream targets. A Western blot results for protein expression of SLC5A7, p53, p53 (ser37) and GAPDH in HCT116 and RKO tumors stably transfected with sgRNA, sgSLC5A7-2, and sgSLC5A7-3. B p21, IGFBP3, CDK6, Bax and GAPDH expression level in HCT116 and RKO tumors stably transfected with sgRNA, sgSLC5A7-2, and sgSLC5A7-3. C–E Immunohistochemical (IHC) staining of SLC5A7 (C), p53 (D), and p53 (ser37) (E) in HCT116 tumors. The percentages of SLC5A7, p53, and p53 (ser37) positive cells were calculated and compared with sgRNA control group (n = 5, **p < 0.01, Student’s t test)