Fig. 4

Anti-tumor effect of targeted demethylation of SLC5A7 in vitro and in vivo. A Cell counting kit-8 (CCK-8) assay was applied to detect cell viability after transfection of sgRNA, sgSLC5A7-2, and sgSLC5A7-3 (n = 5; **p < 0.01, Student’s t test). B Clone formation assay was performed to detect the ability of tumor colony formation after transfection of sgRNA, sgSLC5A7-2, and sgSLC5A7-3. Each well incubated with approximately 1000 cancer cells (n = 3; **p < 0.01, Student’s t test). C Macroscopic images of stable transfection of sgRNA, sgSLC5A7-2, and sgSLC5A7-3 HCT116 tumors. D, E Tumor volume (D) at different time point and tumor weight (E) at killing were monitored. Approximately 5 × 10⁶ HCT116 cells stably transfected with sgRNA, sgSLC5A7-2, and sgSLC5A7-3 were injected in the right flank of female BALB/c nude mice (**p < 0.01, Student’s t test)