Fig. 3
From: Targeted demethylation of the SLC5A7 promotor inhibits colorectal cancer progression
Demethylation of SLC5A7 by dCas9 and sgSLC5A7. A Arrangement of specific demethylation system dCas9-multiGCN4/scFv-TET1CD-sgRNA. The target sites of sgRNAs are illustrated in blue arrowheads. B SLC5A7 mRNA expression determined by qPCR after transfection of dCas9-multiGCN4/scFv-TET1CD-sgRNA and sgSLC5A7(1–6). (Transfection of dCas9-multiGCN4/scFv-TET1CD-sgRNA have only used dCas9-multiGCN4/scFv-TET1CD fragment which do not specific target SLC5A7 site.) C Western blot results showed that SLC5A7 protein expression elevated after transfection of sgSLC5A7-2 and sgSLC5A7-3. D, E DNA methylation analysis of selected CpG sites in SLC5A7 gene after using sgSLC5A7-2 and sgSLC5A7-3 targeted demethylation reagents by time-of-flight mass spectrometry. The dots represent CpG islands and the color of the bar below from green to blue indicates β value range from 0 to 1 (D). SLC5A7 promotor methylation was analyzed (**p < 0.01, compared with sgRNA group, E)