Fig. 2

DM-SSP intervention-associated differential gene expression overlaps DML. a Heatmap of Log₂ (RPM) of the differentially expressed genes (DEGs) by unsupervised hierarchal clustering show two main clusters between baseline (orange) and post-intervention (green). RPM, reads per million reads. b Venn diagram of DML dataset at known genes (blue), DEGs (yellow), and overlap between CpGs-enriched at DEGs in both (purple). c–k Plots display correlation between methylation (x-axis) and gene expression (y-axis) from baseline (orange) and post-intervention (green) for a subset of overlapping CpG-enriched genes (Fig. 3b) annotated to the immune response, metabolic processes, cardiovascular processes, and cardiometabolic diseases, including c ADRB2, d BCL2, e CD6, f DOCK2, g DUSP10, and h FCER2, i LIPA, j NCK1, and k PCNXL2. ADRB2: adrenoceptor beta 2; BCL2: BCL2 apoptosis regulator; CD6: cluster of differentiation 6; DOCK2: dedicator of cytokinesis 2; DUSP10: dual specificity phosphatase 10; FCER2: Fc fragment of IgE receptor II; LIPA: lipase A; NCK1: NCK adaptor protein 1; PCNXL2: pecanex-like protein 2. Significance taken at P value < 0.05