Fig. 1

DM-SSP intervention-associated DNA methylation signatures in monocytes. a Differentially methylated loci (DML) in monocytes from participants at intervention timepoints, baseline (orange) and post-intervention (green), and among non-diabetic controls (red) identify distinct clusters of methylation patterns from unsupervised hierarchical clustering analysis (Manhattan distance, complete linkage method). Methylation values (β-value) are displayed as a range from low (0, blue) to high methylation (1, red). b Plot shows the expected (fuchsia) and observed (blue) CpG probe count for the DML at specific genomic regions (enhancer, promoter, gene body, 3’UTR, and intergenic) and the distribution around CpG Islands (CpG Island, N-shore, S-Shore, N-Shelf, S-Shelf, Open Sea). c Gene ontology analysis of DML enriched at the most significant biological processes indicated. d Differential DNA methylation between intervention timepoints (δβ-value =|Baseline β-value - post-intervention β-value|) at genes annotated to immune response-related functions. P  value of < 0.05, < 0.01, < 0.001, and < 0.0001 indicated by *, **, ***, and ****, respectively. Significance taken at P value < 0.05