Fig. 2

Expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in peripheral blood mononuclear cells (PBMCs). Representative plots showing the gating strategy for separation of lymphocyte and monocyte subsets and the expression of TH and DAT in naïve PBMCs from a donor (A). Lymphocytes and monocytes were determined by side-scatter height (SSC-H) and forward-scatter area (FSC-A). T cells were stained with surface markers CD8 and CD3 and divided into CD8 + and CD8- T cells. Classical, intermediate and non-classical monocytes were defined based on the expression of CD16 and CD14. The percentage of TH⁺ and DAT⁺ double-positive cells was higher in monocytes subsets than in T cell subsets (B). In addition, T cells subsets had a higher percent of cells expressing only TH and a higher percentage of populations were TH⁻ and DAT⁻ double negative than in monocyte subsets. One-way ANOVA followed by Bonferroni post hoc test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n = 6