Fig. 1

Epigenetic silencing of the LINC00473 in colorectal cancer cells. A Bisulfite genomic sequencing analysis of LINC00473 promoter CpG island in the colorectal cancer cell line HCT-116 and normal tissue. Locations of CpG dinucleotides (vertical lines) and the TSS (long black arrow) are shown. Ten single clones are represented for each sample. The presence of unmethylated and methylated CpGs is indicated by white and black squares, respectively. B DNA methylation-associated transcriptional silencing of LINC00473 in the colorectal cancer cell line HCT-116. Expression levels of LINC00473 were determined by qRT-PCR in the methylated cancer cell line HCT-116 and in colorectal normal tissues (N = 3). C Restored LINC00473 expression in the methylated cancer cell line HCT-116 after AZA treatment analyzed by qRT-PCR. Values were determined from triplicates and are expressed as the mean ± SEM. D Methylation and expression analysis of LINC00473 in common colorectal cancer cell lines and normal colon mucosa using 450K array and RNA-seq public datasets. Expression levels obtained by RNA-seq were expressed as transcripts per million (TPMs). TSS, transcription start site; NC; normal colon mucosa