Fig. 3

Abnormal 5hmC enrichment at key TF binding sites in patients with myeloid neoplasms. A Heatmap representation of the 5hmC deviation score at the annotated TF-binding motifs (n = 380). Higher deviation scores represent more enrichment of 5hmC in corresponding TFs motifs; negative deviation scores mean depletion of 5hmC in TF-binding motifs. Each row represents an individual TF motif. Each column stands for an individual case. Red box, 5hmC enrichment status of the indicated C/EBP family members. B The rank of 5hmC changes within the analyzed TF-binding motifs. The C/EBP family members are generally ranked among the top 10 mostly-enriched motifs. C Genome-browser views of the overlaid 5hmC enrichment at the C/EBPβ binding sites. The 5hmC signals within each individual at each cluster were overlaid. C/EBPβ binding sites were obtained from the public C/EBPβ ChIP-seq datasets (GSM2345026 and GSM2345027). D Heatmap representation of 5hmC deviation scores in healthy donors and patients with known TET2 mutation status (WT vs mutation) at the binding motifs of the C/EBP families. E Heatmap representation of the expression of C/EBPβ target genes [46] (n = 527) in the analyzed cohort. The C/EBPβ target genes were defined as genes containing C/EBPβ binding sites within 1-kb of their transcription start site. The C/EBPβ binding sites were identified from public ChIP-seq data (GSM2345026 and GSM2345027). F The t-SNE analysis on the expression of C/EBPβ target genes [46] in healthy donors and patients with AML, CMML or MDS