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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Data mining of bulk and single-cell RNA sequencing introduces OBI1-AS1 as an astrocyte marker with possible role in glioma recurrence and progression

Fig. 1

A In this volcano plot, differentially expressed LncRNAs in GBM compared to LGG are presented. The names of the top 10 with the least FDR are displayed in the figure with OBI1-AS1 having the highest |Log2FC|. Positive values on the x-axis indicate increased expression in LGG vs GBM. B RNA-Seq Analysis of 120 glioma samples revealed decreased of OBI1-AS1 expression in GBM compared to LGG by Log2FC = − 3.67. C Based on survival analysis of 338 glioma patients, those with low expression of OBI1-AS1 exhibited significantly poorer prognosis with Hazard Ratio (HR) = 0.14. D Real-time PCR results confirmed our DEA findings. As is presented, OBI1-AS1 was overexpressed in non-GBM glioma samples by log2FC = − 3.54 which was in accordance with our RNA-Seq data analysis findings (p value < 0.0001). E The ROC curve illustrated AUC equal to 81% and 85% for real-time PCR and RNA-seq data, respectively, with 52 samples (26 for GBM and 26 for LGG) for real-time PCR and 698 for RNA-seq investigation. These AUCs also highlight agreement between RNA-seq and real-time PCR findings. F Expression of OBI1-AS1 decreased after recurrence (p value = 0.005). In 12 of the 14 patient, OBI1-AS1 expression decreased after recurrence

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Clinical Epigenetics

ISSN: 1868-7083

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