Fig. 3

LINC00114 recruits EZH2 to increase the enrichment of H3K27me3 in the DLC1 promoter. A, B RT-qPCR and Western blot detected the mRNA and protein expression levels of DLC1 in Eca109 cells after interfering with LINC00114; C, D RT-qPCR and Western blot detected the mRNA and protein expression levels of DLC1 in TE1 cells after interfering with LINC00114; E, F RT-qPCR and Western blot detected the mRNA and protein expression levels of DLC1 after interference with EZH2 in Eca109 cells; G, H RT-qPCR and Western blot detected the mRNA and protein expression levels of DLC1 after interference with EZH2 in TE1 cells; I Western blot detected EZH2 and H3K27 protein levels after interference with EZH2 in Eca109 cells; J ChIP experiment detected H3K27me3 enrichment of DLC1 promoter in Eca109 cells after interference with EZH2; K Western blot detected EZH2 and H3K27 protein levels in TE1 cells after interference with EZH2; L ChIP experiment detected H3K27me3 enrichment of DLC1 promoter after interference with EZH2 in TE1 cells; Data were shown as mean ± standard deviation. N = 3, in A, B, E, F, I, # P < 0.05 versus the sh-NC group; in C, D, G, H, K, *P < 0.05 versus the sh-NC group. ^ P < 0.05 versus IgG