Fig. 2

LINC00114 binds with EZH2; silencing EZH2 inhibits growth and glycolysis of EC cells. A RNA pull-down experiment observed whether LINC00114 can bind to EZH2; B RIP assay detected the binding between LINC00114 and EZH2; C, D RT-qPCR detected EZH2 expression in tissue samples (EC tissues and non-tumoral tissues) and cells (HEEC and EC cells); E RT-qPCR detected the transfection efficiency of sh-EZH2 in EC cells; F CCK-8 detected proliferation of EC cells after EZH2 interference; G colorimetry detected glucose consumption in EC cells after EZH2 interference; H lactic acid determination kit II detected lactic acid production in EC cells after EZH2 interference; I ATP colorimetric determination kit detected ATP level in EC cells after EZH2 interference; Data were shown as mean ± standard deviation. N = 3; # P < 0.05 versus IgG. & P < 0.05 versus HEEC. *P < 0.05 versus the sh-NC group