Fig. 1

Evaluation of latent versus lytic EBV DNA by methyl-qPCR. Methylation Index was measured on various EBV containing samples. EBV + Akata cell line during latency (A) and reactivation (B). EBV-positive saliva from healthy patients (n = 10) (C). Peripheral blood from patients with primary EBV infection (n = 9) (D); Peripheral blood from confirmed PTLD (post-transplant lymphoproliferative diseases) (n = 8) (E); Peripheral blood from HSCT recipients with no proof of PTLD (n = 5) (F). All samples were submitted to methyl-sensitive qPCR on four distinct regions of the viral genome. Each dot represents one individual sample. Whole extracted DNA were digested with either MspI or HpaII, two isoschizomers with different sensitivities to CpG methylation. HpaII is methylation sensitive, whereas MspI is methylation insensitive. For each region specified above, results are expressed in comparison with non-digested DNA. Data are expressed as mean ± SD