SEEMLIS: a flexible semi-automated method for enrichment of methylated DNA from low-input samples

Clinical Epigenetics

Table 2 Primer and probe sequences used in qPCR and pre-amplification of enriched methylated DNA

Gene	Primer	Sequence (5′–3′)
GSTP1	Forward	TTCGCTGCGCACACTTC
	Probe	CGGTCCTCTTCCTGCTGTCTGTTT
	Reverse	CTTTCCCTCTTTCCCAGGTC
RASSF1	Forward	CCTCCAGAAACACGGGTA
	Probe	TTTGCGGTCGCCGTCGTTGT
	Reverse	CTTCCTTCCCTCCTTCGTC
APC	Forward	TTATTACTCTCCCTCCCACCTC
	Probe	TCTTGTGCTAATCCTTCTGCCCTGC
	Reverse	TGGCAGTTGACACGCATAG
RARB	Forward	GAAGGAGAACTTGGGATCTT
	Probe	TTTCCAGGCTTGCTCGGCCAATC
	Reverse	AGCCTGTAATTGATCCAAATGA
LINE1	Forward	CGCAGGCCAGTGTGTGT
	Probe	CCGTGCGCAAGCCGA
	Reverse	TCCCAGGTGAGGCAATGC

Sequences for forward and reverse primers and internal hydrolysis probe are indicated for each gene. These sequences were used in the design and ordering of custom TaqMan probes for qPCR and pre-amplification of methylated DNA enriched by MBD2-MBD

ISSN: 1868-7083