Fig. 5
From: SEEMLIS: a flexible semi-automated method for enrichment of methylated DNA from low-input samples
Detection of multiple genes in cells selected with single cell aspirator. A Brightfield image of single cell aspirator microwell array used to select individual LNCaP cells. B Representative image of a CTC in a microwell identified by immunofluorescent imaging as positive for Hoechst and anti-cytokeratin and negative for a panel of white blood cell antibodies. C Methylation index for indicated genes for groups of 10–15 CTCs aspirated from 3 patient samples. 10 LNCaP and 10 WBCs included as controls. All error bars represent standard error of the mean (SEM)