Skip to main content
Fig. 7 | Clinical Epigenetics

Fig. 7

From: Atheroprotective mechanism by which folic acid regulates monocyte subsets and function through DNA methylation

Fig. 7

FA suppresses AS plaque formation and reduces the intermediate monocyte proportion in ApoE−/− mice. Mice were divided into seven subgroups: G1, ApoE−WT + ND; G2, ApoE−/− + ND; G3, ApoE−/− + HFD; G4, ApoE−/− + HFD + FA; G5, ApoE−/− + ND + Hcy; G6, ApoE−/− + ND + Hcy + FA; and G7, ApoE−/− + HFD + Hcy + FA. A Representative oil red O-stained and MOMA-immunostained images of G1–G7 mouse aortic sinus cross sections. For immunostaining, cell nuclei were stained with DAPI (blue) and monocytes/macrophages were stained with MOMA antibodies (red). B The severity of vascular lesions was evaluated by determining the ratio of the atherosclerotic plaque lesion area to the luminal area (%). C The accumulation of monocytes/macrophages in atherosclerotic plaques was evaluated by determining the ratio of the MOMA-positive area to the atherosclerotic plaque lesion area (%). D Mouse monocyte subset gating strategy (a) and the proportions of classical (b), intermediate (c), and nonclassical (d) monocytes. E, F Correlation analysis of the proportion of intermediate monocytes and the severity of vascular lesions (blue) and the accumulation of monocytes/macrophages (green) in high fat (G3)- and high Hcy (G5)-treated ApoE−/− mice. Neg., no AS plaque formation; WT, wild type; ND, normal diet; HFD, high-fat diet; FA, folic acid; Hcy, homocysteine. #, the G1 group was significantly different from the other six groups; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Back to article page