Fig. 5
From: A core of differentially methylated CpG loci in gMDSCs isolated from neonatal and adult sources
Assessing uniform change of whole blood DNAm in cancer studies for the 189 consistent loci and the core 10 loci. A Upset plot showing the overlap of the 189 DMRs with consistent gMDSC-Neu delta-betas in adult and cord blood samples and the respective CpG loci in the HNSCC and glioma studies that had case–control delta-betas in the opposite direction (termed “opposite direction CpGs”). B Histogram of the distribution under the null hypothesis of the number of loci with case–control delta-betas that are in the opposite direction of the respective gMDSC-Neu delta-beta in both the HNSCC and glioma study. Distribution was created by randomly drawing 189 CpG loci from the set of all loci with consistent gMDSC-Neu delta-betas and the counting the number of loci that have case–control delta-betas in the opposite direction of gMDSC-Neu delta-betas in both the glioma and HNSCC studies. The horizontal red line is at our observed value of 122 loci, far outside the range of the distribution. C Upset plot of CpGs that were significantly different between cases and cancer-free controls from testing the association between whole blood methylation beta-value and cancer status for each of the gMDSC core loci in two cancer studies. D Heatmap of the whole blood methylation delta-beta for cancer versus control in the glioma study population and the HNSCC study population. Each row represents one of the core ten loci. The color in the heatmap indicates the delta-beta value