Fig. 3
From: A core of differentially methylated CpG loci in gMDSCs isolated from neonatal and adult sources
Identifying putative neutrophil/gMDSC DMRs from adult blood samples and cord blood samples. A Volcano plot of − log 10(q-value) against delta-beta, which represents the difference in mean methylation beta-value between neutrophils and gMDSCs, adjusted for sex, in adult blood samples. The horizontal black bar represents the threshold of significance (q-value (FDR) = 0.05) and the two vertical black bars represent the threshold for delta-beta (delta-beta =  ± 0.2). B Unsupervised clustering heatmap of the top 100 gMDSC DMRs identified in adult blood samples, defined as the 100 CpG loci with the smallest adjusted p-value and absolute adjusted delta-beta greater than 0.2. Each column represents a sample, and each row represents a CpG locus. Above the heatmap, color indicates cell-type (blue = gMDSC and pink = neutrophil). Within the heatmap, color indicates methylation beta-value (blue = β of 1, or methylated, and yellow = β of 0, or unmethylated). C Volcano plot of − log10(q-value) against delta-beta, which represents the difference in mean methylation beta-value between neutrophils and gMDSCs in cord blood samples. The horizontal black bar represents the threshold of significance (q-value (FDR) = 0.05) and the two vertical black bars represent the threshold for delta-beta (delta-beta =  ± 0.2). D Unsupervised clustering heatmap of the top 100 gMDSC DMRs identified in cord blood samples, defined as the 100 CpG loci with the smallest adjusted p-value and absolute delta-beta greater than 0.2. Each column represents a sample, and each row represents a CpG locus. Above the heatmap, color indicates cell-type (blue = gMDSC and pink = neutrophil). Within the heatmap, color indicates methylation beta-value (blue = β of 1, or methylated, and yellow = β of 0, or unmethylated)