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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Bisulfite profiling of the MGMT promoter and comparison with routine testing in glioblastoma diagnostics

Fig. 4

A Average DNA methylation of CpGs covered by MSP primers in samples discordant for MSP-based methylation detection; F, Felsberg primer set; E, Esteller primer set; f,  forward primer; r, reverse primer. B Proposed localization of primers for an optimized MGMT promoter MSP assay; yellow: exon 1; turquoise: neighboring CpGs discriminating between unmethylated and methylated states with lowest logistic regression model p values; pink: forward and reverse primer binding sites, arrows indicate the directionality of the primers; grey: ambiguously called nucleotides (taken from dbSNP153); red: CpG sites, numbered relative to the TSS

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