Fig. 2

Cell-free 5hmC for detection of lung cancer. T-SNE plot (A) and heatmap (B) of 189 lung cancer patients and 157 healthy volunteers based on 3718 differentially hydroxymethylated peaks (DhMPs). Hierarchical clustering was performed across peaks and samples. C Performance of 5hmC model in the training set, validation set and public cfDNA 5hmC data retrieved from Zhang et al. containing 66 NSCLC patients and 67 healthy controls. D Boxplot of the wd-scores calculating with 5hmC model for stage I-IV lung cancer samples. E Genome Browser view of the 5hmC peaks in HAUS3 gene in chromosome 4 shows a marker locating within the gene (boxed region: chr4: 2239149–2239350). AUC, area under the curve; wd-score, weighted diagnosis score