Fig. 1

Chemotherapy induces EMT in murine and human BLBC cells. A Scatter plot showing EMT signatures, using the online pathway enrichment analysis tool gProfiler, on pG-2 cells upon CAF treatment for 48 h. B Brightfield pictures of HCC1806 cells showing a mesenchymal phenotype upon different chemotherapies (CAF, cisplatin and paclitaxel) for 48 h. The yellow arrows indicate cells with pronounced mesenchymal morphology. White scale bars = 50 µm, C Quantitative real-time PCR (qRT-PCR) on EMT-related genes in vehicle- and CAF-, cisplatin- or paclitaxel-treated (for 48 h) HCC1806 cells. All experiments were performed in biological triplicate. ns = not significant, *p-val < 0.05, **p-val < 0.01, ***p-val < 0.005. C Student's t test. Error bars are standard error of the mean (SEM)