Fig. 4

RNA sequencing. A RNA expression changes of all genes. Gray dotted line indicates p = 0.05, red and green dotted lines indicate − 0.7 and 0.7 log(fold change) decrease and increase, respectively. B RNA expression changes of genes that were determined to have the largest promoter region demethylation as determined by methylation analysis. Gray dotted line indicates p = 0.05, red and green dotted lines indicate − 0.7 and 0.7 log(fold change) decrease and increase, respectively. C Histogram and Gaussian distribution curves of the frequency of each degree of RNA expression change between pre- and on-treatment tumors. Gray and pink histogram bars indicate the individual frequencies of each level of RNA expression change in the non-promoter demethylated genes and promoter demethylated genes, respectively. Black and red lines are the Gaussian curves for the non-promoter demethylated genes and the promoter demethylated genes, respectively. Solid black and red bars indicate the 95% confidence intervals of the mean RNA expression change for the non-promoter demethylated genes and the promoter demethylated genes, respectively