Table 4 Primer sequences
Gene | 5′ to 3′ | Tm °C | |
|---|---|---|---|
AJAP1 | M | F: TTTGGTAGAGTTTTTCGATTCGGTAGC R: ACCGAAACTCCGCGCCGATAA | 60 |
U | F: TTTGGTAGAGTTTTTTGATTTGGTAGT R: CCAAAACTCCACACCAATAA | 55 | |
CDH1 | M | F: TTAGGTTAGAGGGTTATCGCGT R: TAACTAAAATTCACCTACCGAC | 57 |
U | F: TAATTTTAGGTTAGAGGGTTATTGT R: CACAACCAATCAACAACACA | 55 | |
CDH13 | M | F: TCGCGGGGTTCGTTTTTCGC R: GACGTTTTCATTCATACACGCG | 57 |
U | F: TTGTGGGGTTTGTTTTTTGT R: ACATTTTCATTCATACACACA | 53 | |
MAGI2 | M | F: CGTAGAGTTCGAGATGTGGTATTAGGC R: AAACTCCTATACGAAAAAAACGCGCTA | 60 |
U | F: TGTAGAGTTTGAGATGTGGTATTAGGT R: AACTCCTATACAAAAAAAACACACTA | 55 | |
MGMT | M | F: TTTCGACGTTCGTAGGTTTTCGC R: GCACTCTTCCGAAAACGAAACG | 59 |
U | F: TTTGTGTTTTGATGTTTGTAGGTTTTTGT R: AACTCCACACTCTTCCAAAAACAAAACA | 59 | |
MYOD1 | M | F: GACGGTTTTCGACGGTTT R: GCCCGAAACCGAATACAC | 56 |
U | F: ATTTGATGGTTTTTGATGGTTT R: CACACACATACTCATCCTCACA | 57 | |
RASSF1A | M | F: GTGTTAACGCGTTGCGTATC R: AACCCCGCGAACTAAAAACGA | 65 |
U | F: TTTGGTTGGAGTGTGTTAATGTG R: CAAACCCCACAAACTAAAAACAA | 60 | |
SOX17 | M | F: GGAGATTCGCGTAGT TTTCG R: AACCCGACCATCACCGCG | 60 |
U | F: GGAGATTTGTGTAGT TTTTG R: ACCCAACCATCACCACA | 55 | |
GAPDH | M | F: GAGAAAGTAGGGTTCGGTTATTAGC R: AAAAACGAAACGAAAAACTACGA | 55 |
OXT | M | F: ATAAAAAGGTTAGGTCGGAGAGATC R: AAATATAACAAACGAAAATCAACGC | 52 |