Fig. 3

Severe acute pancreatitis-predicting MHBs were identified based on their average uMHL scores in cfDNA samples. A 59 MHBs whose average uMHL scores were lower in MAP samples than in SAPs’ samples were identified as SAP classifiers. Heatmap visualizes the differences in the uMHL scores of those MHBs between MAP and SAP samples in the training set; samples were arranged by each patient and by days; B swarm plot of the aggregate uMHL scores of the 59 MHB sites robustly separate MAP and SAP plasma samples of either training or test sample set; C SAP prediction accuracy by the aggregated uMHL scores of the 59 MHBs on test set samples. SAP, severe acute pancreatitis; cfDNA, cell free DNA; MAP, mild acute pancreatitis; MHB, methylated haplotype block; uMHL, unmethylated haplotype load