Fig. 2

Acute pancreatitis-predicting MHBs were identified based on their uMHL scores in cfDNA samples. A 565 MHBs that were hypermethylated in healthy individuals’ cfDNA samples but relatively hypomethylated in APs’ samples were identified as classifiers for AP plasma. Heatmap visualizes the differences in the uMHL scores of those MHBs between healthy controls and AP samples in the training set; samples were arranged by each patient and by days; B swarm plot of the aggregate uMHL scores of the 565 MHB sites shows that they robustly separated healthy and AP plasma samples of either training or test set; C AP prediction accuracy by the (aggregated) uMHL scores of the 565 MHBs on test set AP samples over healthy controls; D Genes associated with the 565 identified AP markers are enriched in pancreas- and kidney-related Gene Ontology categories. AP, acute pancreatitis; cfDNA, cell free DNA; MHB, methylated haplotype block; uMHL, unmethylated haplotype load