Fig. 2From: High performance methylated DNA markers for detection of colon adenocarcinomaStudy design. Fresh frozen samples of colon carcinoma and normal colon tissues were randomized into training and test sets matched for patient age. Thirteen markers selected in Fig. 1 were quantitatively evaluated for DNA methylation using the QM-MSP assay in samples from the training set (N = 53). A subset of markers was then selected based on high methylation in carcinoma and low methylation in normal tissue adjacent to tumor (Adj. Normal). A 13-gene and a 6-gene panel were evaluated in the test cohort (N = 52). To explore the possibility that these markers were useful in liquid biopsy, a pilot study of plasma samples from colorectal carcinoma patients (N = 20) and normal individuals (N = 20) was tested for the presence of cell-free plasma DNA using the cMethDNA assayBack to article page