Fig. 4

Genome-wide DNA methylation signature difference between pregnant and non-pregnant adrenocortical adenomas. A, B Heatmap of DNA methylation in adrenocortical adenomas in non-pregnant (A) and pregnant (B) patients based on genome-wide DNA methylation status. Heat maps are used to compare the similarity and difference of the two groups. For drawing the figures, top 5000 differences DMPs were chosen; if the differences DMPs sites were less than 5000, all differences will be displayed. The results of the heatmap drawn directly using the value shown on the left side of the figure. C, D Scatter plots are used to show the difference in genome-wide DNA methylation between the C and D groups. The red points denote points mean Δβ > 0.2, and the green points denote points mean Δβ < 0.2. E, F TSS methylation distribution map of non-pregnant (E) and pregnant groups (F). We extracted the relevant information from differential methylation sites and compared the methylation differences between groups of 5 kb upstream and downstream of the transcription start site TSS. G The genome-wide DNA distribution of hypermethylated and hypomethylated DMPs in non-pregnant and pregnant groups. H The chromosomal distribution of methylated DMPs in non-pregnant (orange) and pregnant (blue) groups