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Table 2 Cord blood differentially methylated positions associated with Mn and Pb (FDR < 0.05)

From: Prenatal metal exposure, cord blood DNA methylation and persistence in childhood: an epigenome-wide association study of 12 metals

CpG Chr Positiona Gene Feature category Cord blood Mid-childhood
      p FDR Median % methylation Mean difference in % methylation (95% CI) p Median % methylation Mean difference in % methylation (95% CI)
Mn            
 All infantsb            
 cg02042823 16 6,714,429 A2BP1 5'UTR 2.47 × 10–11 9.73 × 10–6 98.29 2.65 (2.07, 3.23) 0.010 98.65 0.75 (0.14, 1.36)
 Femalec            
  cg00954161 1 3,696,925 LRRC47 3'UTR 2.23 × 10–4 0.037 98.5 1.54 (1.08, 2.01) 0.001 98.41 1.33 (0.69, 1.98)
  cg11161853 3 67,705,044 SUCLG2 TSS200 1.03 × 10–6 0.048 2.43 − 0.32 (− 0.43, − 0.22) 0.73 2.37 0.01 (− 0.06, 0.07)
  cg23903787 4 3,527,371 LRPAP1 Body 3.13 × 10–7 0.038 89.44 4.60 (3.32, 5.88) 0.011 89.48 2.04 (0.79, 3.29)
  cg19908812 4 164,253,006 NPY1R 5'UTR 9.36 × 10–7 0.048 1.47 − 0.30 (− 0.38, − 0.21) 0.55 2.90 − 0.03 (− 0.34, 0.27)
  cg26462130 7 2,052,961 MAD1L1 Body 1.10 × 10–6 0.048 98.63 2.04 (1.67, 2.41) 0.005 98.71 0.98 (0.68, 1.28)
  cg08904630 10 71,490,427   9.42 × 10–7 0.048 97.74 0.84 (0.64, 1.04) 1.27 × 10–5 97.93 0.48 (0.34, 0.62)
  cg22799518 12 56,988,862 RBMS2 3'UTR 3.80 × 10–7 0.037 98.80 2.03 (1.62, 2.44) 0.004 98.74 − 0.20 (− 0.43, 0.03)
  cg01744822 16 73,100,510   9.19 × 10–7 0.048 4.59 − 1.16 (− 1.45, − 0.86) 9.44 × 10–5 8.89 − 1.66 (− 2.29, − 1.03)
  cg15712310 16 73,100,790   1.93 × 10–7 0.037 24.87 − 2.69 (− 3.56, − 1.81) 0.039 36.01 − 0.97 (− 1.86, − 0.07)
 Maled            
  cg03763518 1 150,245,044 C1orf54 TSS200 4.75 × 10–8 0.009 7.62 − 3.01 (− 3.72, − 2.30) 1.74 × 10–4 32.41 − 3.03 (− 4.56, − 1.49)
  cg02042823 16 6,714,429 A2BP1 5'UTR 2.79 × 10–10 1.10 × 10–4 98.22 3.40 (2.73, 4.07) 2.34 × 10–4 98.65 1.82 (0.51, 3.13)
Pb            
 All infants b            
  cg20608990 2 202,097,607 CASP8 TSS1500 1.02 × 10–7 0.040 64.54 − 3.10 (− 4.22, − 1.97) 0.008 48.64% − 1.56 (− 2.73, − 0.40)
  1. Differentially methylated positions (DMPs) identified using models of individual log2-transformed prenatal metal concentration adjusted for infant sex, race/ethnicity, gestational age (age at blood collection for mid-childhood models), nulliparous, maternal age at enrollment, pre-pregnancy BMI, education, smoking, household income, and estimated cord blood cell-type proportions (estimated in cord blood or from leukocytes in mid-childhood). Mean difference in % methylation determined using the same adjusted models for Beta-values for interpretation of results
  2. ahg 19 assembly
  3. bCord blood: N = 361; mid-childhood: N = 333
  4. cFemale (Cord blood: N = 169; mid-childhood: N = 158)
  5. dMale (Cord blood: N = 192; mid-childhood: N = 175)