Fig. 4

Viability and cell cycle analysis using siRNA-mediated knockdown of GSTM1 showed that knockdown of GSTM1 decreased cell viability and caused cell cycle arrest in U87 cells. a Knockdown of GSTM1 showed a significant reduction of GSTM1 mRNA expression by RT-PCR assays. Data from three independent experiments were expressed as mean ± s.d. (s.d.: standard deviation). The asterisk (*) indicated statistical significance (P < 0.05) between GSTM1 siRNA and scrambled siRNA groups. b Knockdown of GSTM1 decreased the percentage of viable U87 cells down to 84.3%, compared to control knockdown cells at 48 h. Data from three independent experiments were expressed as mean ± s.d. The asterisk (*) indicated statistical significance (P < 0.05) between GSTM1 siRNA and scrambled siRNA groups. c The cell cycle profile showed that knockdown of GSTM1 caused G0/G1 cell cycle arrest after GSTM1 was knocked down at 48 h. d The percentage of G0/G1 cells was significantly increased in GSTM1 siRNA knockdown U87 cells compared to scrambled siRNA U87 cells. Data from three independent experiments were expressed as mean ± s.d. A bar graph containing different cell cycle phases (G0/G1, S, G2/M) was shown on the right